Interleukin17A (IL-17A) is disulfide-linked homo dimeric cytokine of 155 amino acids and a member of an IL-17 family of related cytokines. There is now compelling evidence that patients affected by autoimmune diseases have a higher incidence of several cardiovascular diseases. In this study, we validate a method to quantify IL-17A in human serum using the ultrasensitive Quanterix Simoa™ platform.

An Ultrasensitive LC-APPI-MS/MS Method for Simultaneous Determination of Ciclesonide and Active Metabolite Desisobutyryl-Ciclesonide in Human Serum and Its Application to a Clinical Study

Oligonucleotide (OGN) therapy is increasingly recognized as an important therapeutic modality with the promise of curing diseases. However, unlike most biotherapeutics, the development of OGN drugs often requires their determination with high sensitivity and specificity in multiple, complex biologic matrices such as blood and tissues in order to measure their exposure and distribution for understating PK, PD, and safety.

At Frontage Laboratories (PA, USA), we have developed highly sensitive and specific quantitative methods for the determination of OGN in plasma and tissues using hybridization immunoassay and LC–MS techniques. In this webinar, we will present the method development, validation, and comparison of these techniques for the quantification of OGN as well as a case study with antisense OGN.