- Metabolic Stability
- CYP Interaction
- Permeability & Transporters
- Physicochemical Properties
- Protein Binding
Discovery PK and Toxicology
Frontage offers multiple compound stability services to help elucidate the metabolic profile of your drug. Metabolic stability or clearance is recognized as one of the main determinants of drug concentration in blood and has been used effectively to predict pharmacokinetics and toxicokinetics. Compounds with poor metabolic stability are exposed to comprehensive first-pass metabolism in the liver and gut right after absorption from the gut via the portal vein, causing poor overall bioavailability to circulation and rapid elimination from the body.
In vitro studies on metabolic stability are conducted to measure the rate of disappearance of the molecule using liver microsomes, S9 fraction, or isolated hepatocytes. The in vitro data are valuable in predicting the in vivo hepatic clearance and estimating bioavailability and systemic clearance.
Frontage offers a variety of metabolic stability assays to determine the in vitro half-life and intrinsic clearance of test compounds, with customizable assay conditions. Our assays can be conducted using different matrices from various species. We also offer metabolic stability assays in various buffers, such as simulated intestinal and gastric fluids.