Chiral separation is important but also challenging for bioanalysis of the antifungal drug luliconazole. Since typical reverse phase chromatography conditions used in electrospray or APCI- MS/MS could not achieve the required separation of the 4 enantiomers, we employed APPI (Atmospheric Pressure Photo Ionization) conditions instead.

In this study, we discuss the development and validation of an LC-MS/MS method for the quantification of 15 bile acids in the human serum.

An Ultrasensitive LC-APPI-MS/MS Method for Simultaneous Determination of Ciclesonide and Active Metabolite Desisobutyryl-Ciclesonide in Human Serum and Its Application to a Clinical Study