Development and Validation of an Ultra Sensitive LC-MS/MS Method for Quantitation of Midazolam in Human Plasma

Midazolam is a widely used central nervous system depressant. It is used for the treatment of insomnia, seizure, and induction of sedation or amnesia for operations. It is metabolized by cytochrome CYP3A and is a widely used probing drug for the evaluation of CYP3A activity in drug-to-drug interaction (DDI) studies. In this study, we develop a method to quantify midazolam in human plasma.

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