Determination of Pregabalinin Human Plasma by Liquid Chromatography-Tandem Mass Spectrometry

Pregabalin, (S)-3-(aminomethyl)-5-methylhexanoic acid, is an analog of the neurotransmitter gamma amino butyric acid (GABA), used for the treatment of peripheral and central neuropathic pain in adults and as adjunctive therapy for refractory partial seizures. The aim of this study is to develop a sensitive, specific, robust, and rapid LC-MS/MS method, using a simple extraction procedure with no interference from the matrix and with a short run time.