Comparison of the Quantitative Measurement of Albumin in Human…

Human serum albumin (HSA) is the most abundant protein in human blood plasma. It is comprised of 585 residues with 17 disulfide bonds. Cys34 is its single free thiol group which is thought to act as an oxidant scavenger. Previous chromatographic studies have shown that the HSA-SH fraction of HSA decreases with age; 76% vs. 48%, young vs elderly healthy male subjects, respectively.1,2 The lower percent of SH present is a measure of the frailty of the person. Since ELISA can only measure total albumin, it does not provide a complete assessment of the person’s overall health. Although typical serum albumin levels range from 35-50 mg/mL of serum, lower or higher levels of certain serum albumins indicate disease states (e.g., Cirrhosis of the liver, severe dehydration-kidney disease) which requires treatment. Thus test kits that simply measure the total amount of HSA present in blood plasma may not completely describe the patient condition regarding a measure of frailty of the patient. This poster presents the ability of using LC coupled to high resolution mass spectrometry (HRMS) for the analysis of the different intact albumin proteins.

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