In this infographic, we describe various mass spectrometry-based strategies Frontage has successfully applied in the bioanalysis of ADCs for clinical and preclinical studies. Antibody-drug conjugates (ADCs) combine the best of two modalities: toxin efficacy and antibody specificity. The result is an improved therapeutic window with fewer side effects but the bioanalytical requirements for PK analysis also require a multifaceted approach.

Chiral separation is important but also challenging for bioanalysis of the antifungal drug luliconazole. Since typical reverse phase chromatography conditions used in electrospray or APCI- MS/MS could not achieve the required separation of the 4 enantiomers, we employed APPI (Atmospheric Pressure Photo Ionization) conditions instead.