- Metabolic Stability
- CYP Interaction
- Permeability & Transporters
- Physicochemical Properties
- Protein Binding
Discovery PK and Toxicology
At Frontage, we routinely perform accurate, precise, and ultrasensitive quantification ddPCR assays for DNA and RNA to support several applications, including pharmacokinetics (e.g., DNA, mRNA, and oligonucleotide drugs), pharmacodynamics (e.g., gene expression analysis), pharmacogenomics (e.g., mutations and DNA copy number variation), safety (e.g., viral shedding, biodistribution, etc.) and microbial quantification.
ddPCR (droplet digital PCR) is a digital PCR method based on water-oil emulsion droplet technology. A small (~22 μL) reaction mix is fractionated into ~20,000 droplets, such that the vast majority of droplets contain either zero or one copy of the template. PCR amplification occurs in each individual droplet during a standard PCR reaction. After the reaction, each droplet is analyzed to determine whether amplification of the template occurred, and the result is then converted to the template copy number in the original reaction mix using a Poisson Distribution Analysis. ddPCR results thus provide direct quantifications of templates as standard curves are not needed.
Our labs offer cost-effective examination of oncogenes from tumor tissue, bioﬂuids, or genomic or cfDNA samples using targeted sequencing of oncopanels supported by industry leading manufacturers.Learn More
Frontage offers advanced sequencing technologies to enable profiling of the microbiome DNA composition (metagenomics) and the microbiome RNA composition (metatranscriptomics) at unprecedented scales.Learn More
Our RNA sequencing services use a combination of state-of-the-art equipment that provides quick and accurate results for your RNA profiling projects.Learn More