WRIB 2024: High-quality RNA-seq Library Preparation from Formalin-Fixed Paraffin-Embedded (FFPE) tissues with GLP and GCLP Compliance

Here, we standardized the RNA extraction method from FFPE slides and an RNA-seq operation method under Good Lab Practice (GLP) standards, which have been consistently able to get high-quality data, particularly for degraded RNA (DV200<20%) from FFPE. We also presented some key skills, including quantification, library kit selection, library construction and additional post-PCR process. Our research will enable FFPE sample materials to be used in the NGS without considering materials quality and the NGS will be used in more fields.

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